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Registros recuperados: 5
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Acquisition de données complémentaires aux dénombrements, avec les techniques de cytométrie en flux, fluorescence totale ou spectrale. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° A III. Rapport final, 23 septembre 2014 ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Barthelemy, Vanille; Bonato, Simon; Broutin, Mathias; Lizon, Fabrice; Lefebvre, Alain.
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel-Atlantic zone. The construction of a composition indice must consider the diversity of all the phytoplankton. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance when estimating the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyses pigmentaires; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry; Spectral fluorescence; Pigments analysis.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47259/47224.pdf
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Comparaison des résultats obtenus avec différentes techniques d’évaluation du phytoplancton antérieurement à 2013. Action 3. Indice Composition. Livrable n° A II. Rapport final, 13 novembre 2014. ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Lampert, Luis; Broutin, Mathias; Bonato, Simon; Lizon, Fabrice; Lefebvre, Alain.
L'élément de qualité phytoplancton doit être évalué dans le cadre de la Directive Cadre sur L’Eau grâce à trois indices : biomasse, abondance et composition. Les deux premiers indices, biomasse et abondance, ont déjà été définis. L’indice composition a quant à lui fait l'objet d'une proposition précise en Méditerranée, mais pas en Manche - Atlantique. Les travaux antérieurs montrent que la construction d'un indice de composition doit tenir compte de la diversité de l'ensemble du phytoplancton. Or, les comptages microscopiques ne concernent en général que le micro-phytoplancton. Il est donc nécessaire de prendre en compte des informations supplémentaires qui tiennent compte du nano- et du picophytoplancton, dont l'importance dans la diversité du...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyse CHEMTAX; Projet DYMAPHY.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47258/47223.pdf
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Phytoplankton distribution from Western to Central English Channel, revealed by automated flow cytometry during the summer-fall transition ArchiMer
Louchart, Arnaud; Lizon, Fabrice; Lefebvre, Alain; Didry, Morgane; Schmitt, François G.; Artigas, Luis Felipe.
Automated pulse shape-recording flow cytometry was applied to address phytoplankton spatial distribution, at high frequency, in stratified and well mixed water masses in the Western and Central English Channel during the summer-fall transition. Cytometric pulse shapes derived from optical features of single cells allowed the characterization of eight phytoplankton groups. Abundance and total red fluorescence (chlorophyll a autofluorescence) per group were used to define six phytoplankton communities. Their distribution revealed high spatial heterogeneity. Abundance presented a longitudinal gradient for six over the eight groups and succession of brutal shifts along the cruise. Maximum values were often located near the Ushant front in the Western English...
Tipo: Text Palavras-chave: English Channel; Phytoplankton distribution; High resolution; Automated flow cytometry; Fronts; Mesoscale structure.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71324/69752.pdf
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Premières propositions pour un indice de composition du phytoplancton, basé sur les résultats de cytométrie en flux. Action 3 - Indice Composition - Livrable 2. Rapport final, mars 2016 ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Prevost, Emilie; Bonato, Simon; Lefebvre, Alain.
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel - Atlantic zone. The construction of a composition index must consider the diversity of all the phytoplankton organisms. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance in the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Indice de composition; Cytométrie en Flux de type Scanning; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry Phytoplankton composition index index.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00361/47264/47229.pdf
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Utilisation conjointe de FlowCAM / ZooPhytoImage et de la cytométrie en flux. Premiers résultats et perspectives. Action 9. FlowCam ZooPhytoImage. Livrable n° 4. Rapport final, 23 septembre 2014 ArchiMer
Ali, Nour; Wacquet, Guillaume; Didry, Morgane; Hamad, Denis; Artigas, Luis Felipe; Grosjean, Philippe.
The goal of this study is to investigate about the possibility of coupling measurements made by image analysis from the FlowCAM with Zoo/PhytoImage with data obtained with a flux cytometer (pulse-shape-recording Scanning Flow Cytometry) on the same samples gathered in current monitoring networks in the eastern Channel and southern North Sea. In this preliminary study, we collected a series of samples off Boulogne-sur-Mer (SRN-REPHY monitoring system run by IFREMER) and along a transect in the Baie St-Jean (Wimereux-Slack) run by LOG. All these samples were digitized with a FlowCAM and measured with a scanning flow cytometer (CytoSense). The complete analysis with the FlowCAM and Zoo/PhytoImage is detailed in the present report. In order to get a better...
Tipo: Text Palavras-chave: Manche – Mer du Nord; Phytoplancton; Analyse d'image; Classification supervisée; Cytométrie en flux; Eastern English Channel and southern North Sea; Phytoplankton; Image analysis; Machine learning; Scanning Flow Cytometry.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00363/47442/47470.pdf
Registros recuperados: 5
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